PPAR alpha Antibody, Unconjugated, Rabbit, Polyclonal Preis auf Anfrage

Product Overview

• Article Name: PPAR alpha Antibody, Unconjugated, Rabbit, Polyclonal Preis auf Anfrage
• Biozol Catalog Number: BYT-ORB345414
• Supplier Catalog Number: orb345414
• Alternative Catalog Number: BYT-ORB345414-25
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: PPAR alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Ppar alpha antibody, Pparalpha, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, Ppar-a, Nr1c1, Ppar

Product Description

PPAR alpha antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL
• NCBI: 31543500
• UniProt: P23204
• Buffer: 0.01% (w/v) Sodium Azide
• Purity: This affinity purified antibody is directed against mouse PPAR alpha protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, rat, bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from human, chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: 1:8,000 - 1:32,000, IHC: 1:100-1:300, WB: 1:500 - 1:2,000
• Application Notes: Application Notes: Anti-PPAR alpha Antibody has been tested in ELISA, Western Blot, Immunohistochemistry, and Immunofluorescence. Expect a single band approximately 52 kDa in size corresponding to PPAR alpha by western blot in the appropriate tissue or cell lysate. A 1:200 dilution is suggested for Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user

Images

Adipopnectin regulates the expression of ADAM10 and Notch1 through PPARalpha and JNK pathway respectively. (a) Representative immunoblots and quantification of hippocampal protein levels after chronic ICV injection of AdipoRon, WY14643 (PPARalpha agonist) and GW6471 (PPARalpha antagonist) in 12-month-old mice, including Notch1 and ADAM10. Control, n = 6, AdipoRon, n = 6, WY14643, n = 7, AdipoRon + GW6471, n = 7. *p < 0.05, **p < 0.01, ***p < 0.001 compared with Control, p < 0.05, p < 0.001 compared with AdipoRon treatment. (b) Co-immunoprecipitation results showing chronic ICV injection of AdipoRon or WY14643 enhances the interaction between PPARalpha and RXR. Control, n = 6, AdipoRon, n = 6, WY14643, n = 7. ***p < 0.001 compared with Control. (c) qPCR results showing chronic ICV injection of AdipoRon or WY14643 Induces the recruitment of RXR to the ADAM10 Promoter. Control, n = 6, AdipoRon, n = 7, WY14643, n = 7. ***p < 0.001 compared with Control. (d) Chronic ICV injection of AdipoRon upregulates the activity of JNK. Control, n = 6, AdipoRon, n = 6. ***p < 0.001. (e) Representative immunoblots and quantification of hippocampal protein levels after chronic ICV injection of AdipoRon, Vinblastine (JNK agonist) and SP600125 (JNK antagonist) in 12-month-old mice, including Notch1 and ADAM10. Control, n = 6, AdipoRon, n = 7, Vinblastine, n = 7, AdipoRon + SP600125, n = 7. **p < 0.01, ***p < 0.001 compared with Control, p < 0.001 compared with AdipoRon treatment. Data are presented as means SEM.

Affinity Purified Anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 µg of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.

ELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB ELISA Peroxidase Substrate (p/n orb348651).

Immunofluorescence microscopy of Rabbit Anti-PPAR alpha (N-terminal specific) antibody using (A) Mouse NIH/3T3 or (B) Human HEK293 cells fixed with MeOH. (C) Secondary antibody only with NIH/3T3 cells. Anti-PPAR alpha antibody was used at 10 µg/ml, 1h at RT°. Secondary antibody: Anti-RABBIT IgG DyLight(TM) 488 Conjugated Preadsorbed at 5 ug/ml for 1 h at RT. Staining: PPAR as green fluorescent signal with DAPI (blue) nuclear counterstain.

Immunofluorescence Microscopy of Rabbit anti-PPAR alpha antibody. Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 µg/ml overnight at 4C. Secondary antibody: Alexa Fluor 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) showing Biorbyts PPAR alpha antibody staining of PPAR alpha protein in mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plu