p35 Control protein, Unconjugated Preis auf Anfrage

Product Overview

• Article Name: p35 Control protein, Unconjugated Preis auf Anfrage
• Biozol Catalog Number: BYT-ORB345965
• Supplier Catalog Number: orb345965
• Alternative Catalog Number: BYT-ORB345965-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: SDS-PAGE, WB
• Conjugation: Unconjugated
• Alternative Names: bba64, Borrelia burgdorferi p35, control protein

Product Description

p35 Control protein

Product Properties

• Concentration: 1 mg/ml
• NCBI: 010256558
• UniProt: O50687
• Buffer: 0.01% (w/v) Sodium Azide
• Source: Borrelia burgdorferi
• Purity: p35 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified p35 and was estimated to be greater than 90% pure.
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: User Optimized, WB: User Optimized
• Application Notes: Biological Origin: Borrelia burgdorferi. Application Notes: p35 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 69.5 kDa for p35-MBP, (27.1 kDa for p35 and 42.4 kDa for MBP) in size corresponding to p35 by Western blotting in the appropriate cell lysate or extract. p35 kDa protein has been tested in SDS-page

Images

Antigen-specific IgG2, IgA1, and ADCD partitioning between compartments differs significantly across disease phenotypes. (A) Fraction of samples with non-zero measurements for ADCD, IgG1, IgG2, IgG3, and IgA1 for refractory (dark blue) and responsive (green) patients in the serum (dashed line) and joint fluid (solid line) for each antigen. Significant differences in distribution of non-zero measurements between fluids as assessed by a Fishers exact test are denoted as *p < 0.05, **p < 0.01, ***p < 0.001 for refractory (dark blue) and responsive (green) samples after correction for multiple hypothesis testing via Benjamini-Hochburg. (B) Ratio of fraction of serum samples with non-zero measurements to fraction of joint fluid samples with non-zero measurements for ADCD, IgG1, IgG2, IgG3, and IgA1 for refractory (dark blue) and responsive (green) patients for each antigen. Significant differences in distributions of ratios between phenotypes are assessed by a Mann-Whitney nonparametric test, then corrected for multiple hypothesis testing via Benjamini-Hochburg. CRASP1, CRASP2, DbpA, DbpB, Arp37, flagellin, OspA, OspB, OspC, OspE, p27, p35, p39, VlsE: Biorbyt antigens.

SDS PAGE Results of p35 Control Protein. Lane 1: p35 Control Protein 1X Reduced. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: p35 Control Protein 1X Non-Reduced. 4-20% Gel, Coomassie Stained.

SDS-Page results of p35 Control Protein. Lane 1: p35 Control Protein Reduced [5 µg]. Lane 2: p35 Control Protein Reduced [1 µg]. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: p35 Control Protein Non-Reduced [5 µg]. Lane 5: p35 Control Protein Non-Reduced [1 µg]. 4-25% Gel, Coomassie Stained. p35 Control Protein is a fusion protein with MBP and the expected ~69.5kDa.

Systems serology profiling with Borrelia-specific antigens reveals patient heterogeneity. The heatmap shows the Z-scored measurements for 12 features, across 16 antigens for both refractory and responsive patients, visualized with joint fluid measurements in the upper half of the heatmap and serum measurements in the lower half of the heatmap. Only antigens detected above background for at least 30% of samples were included for each measurement. Statistical significance was assessed using the Mann-Whitney nonparametric test, with p values then corrected for multiple hypothesis testing via Benjamini-Hochburg, *p < 0.05, **p < 0.01, ***p < 0.001, else not significant. CRASP1 (p/n orb345971), CRASP2 (p/n orb345972), DbpA (p/n orb345961), DbpB (p/n orb345969), Arp37 (p/n orb345962), flagellin (p/n orb345967), OspA (p/n orb345966), OspB (p/n orb345968), OspC (p/n orb345964), OspE (p/n orb345963), p27 (p/n orb345973), p35 (p/n orb345965), p39 (p/n orb345970), VlsE (p/n orb345974).