Mouse IgG F(c) Fragment Biotin Conjugation, Mouse IgG Fc Fragment Biotin Conjugated
Mouse IgG F(c) Biotin Antibody
Concentration:
1.0 mg/mL
Buffer:
0.01% (w/v) Sodium Azide
Source:
Mouse
Purity:
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(ab)2 or anti-Papain.
Form:
Lyophilized
Application Notes:
Biological Origin: Mouse. Application Notes: Mouse IgG F(c) fragment has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments
Dot Blot of Mouse IgG F(c) Fragment Biotin Conjugated. Row: Mouse IgG F(c) Biotin Conjugate [1.5 mg/ml]. Column Dilutions: 100 ng, 33.33 ng, 11.11 ng, 3.70 ng, 1.23 ng. Primary Antibody: None. Secondary Antibody: Streptavidin Peroxidase (p/n orb348767) at 1:40000 for 30 mins at RT. Block: BlockOut buffer (p/n orb348644). Exposure: 2 sec.
SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). MW: 5 µl Opal Prestained Marker. Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 2: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 3: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 4: Mouse IgM Kappa Myeloma Protein. Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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