Low Endotoxin Control Rabbit IgG Antibody, Unconjugated Preis auf Anfrage
Biozol Catalog Number:
BYT-ORB346306
Supplier Catalog Number:
orb346306
Alternative Catalog Number:
BYT-ORB346306-500
Manufacturer:
Biorbyt
Category:
Proteine/Peptide
Application:
ELISA
Conjugation:
Unconjugated
Alternative Names:
Rabbit immunoglobulin G, Low EU Immunoglobulin control, Low EU standard
Low Endotoxin Control Rabbit IgG Antibody
Concentration:
1.0mg/mL
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Rabbit
Purity:
Low Endotoxin Control Rabbit IgG is an IgG preparation of whole rabbit serum purified by protein A chromatography using a low endotoxin methodology.
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Biological Origin: Rabbit. Application Notes: Low Endotoxin Control Rabbit IgG has been tested in ELISA and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments
Changes of body temperature (C) over time (h) of rats treated intraperitoneally at 7:00 with TNF-alpha antibodies (50 µg/rat i.p.) and at 8:00 with AUDA in a dose of 15 mg/kg an hour before LPS (50 µg/kg i.p.) (open circles) or NaCl (open squares) administration. Closed triangles represent Tb of rats treated at 7:00 with IgG (50 µg/rat i.p.) and at 8:00 with AUDA an hour before LPS injection (both in same concentration as above). Closed circles represent normal circadian rhythm of body temperature in non-treated rats. Sample size is indicated in parentheses. Black arrowheads represent the time of injection. Values are means SEM at 30-min averages. Asterisks indicate significant difference (***p < 0.001) between experimental groups TNFab/AUDA/LPS and IgG/AUDA/LPS. Low Endotoxin Control Rabbit IgG (p/n orb346306).
Negative controls of neutralization assay. Low endotoxin isotype control Abs were added (goat IgG, 100 µg/ml, rabbit IgG, 2 µg/ml, mouse IgG, 1 µg/ml) to the 10% PRP supplemented media as controls. No significant changes in proliferation were noticed when adding the isotype control Abs to the PRP supplemented hMDPC cultures compare to the PRP groups (n = 4). Low Endotoxin Control Rabbit IgG (p/n orb346306).
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