Rabbit IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rabbit IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Rabbit IgG and anti-Rabbit IgG F(c). No reaction was observed against anti-Rabbit IgG F(ab)2 or anti-Papain.
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Biological Origin: Rabbit. Application Notes: Rabbit IgG F(c) Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments
SDS PAGE gel of commercially-purified rabbit Immunoglobulin G (rIgG) and of rIgG papain digestion products Fab and Fc. Lanes C-F contain products which have been reduced with b-mercaptoethanol. Lanes H-L contain non-reduced products. Each lane contains approximately 35 mg of the following products: Lanes C and H contain papain digested products, Lanes D, I and L contain Fc fragment obtained from protein A purification, Lanes E and J contain whole rIgG, Lanes F and K contain Fab fragment obtained from protein A purification, Lanes A, B and G contain gel markers. Whole rIgG (p/n orb2652747), rFc (p/n orb346310), rFab (p/n orb2652745), rF(ab)2 (p/n orb346311).
SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n orb346310). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n orb346313). Load: 1 µg for F(ab) and F(c), 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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