Rabbit IgG F(ab)2 Antibody, Unconjugated

Product Overview

• Article Name: Rabbit IgG F(ab)2 Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB346311
• Supplier Catalog Number: orb346311
• Alternative Catalog Number: BYT-ORB346311-2
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: SDS-PAGE
• Conjugation: Unconjugated
• Alternative Names: Rabbit Immunoglobulin IgG F(ab)2 fragment, IgG Fab2

Product Description

Rabbit IgG F(ab)2 Antibody

Product Properties

• Concentration: 2 mg/mL
• Buffer: 0.01% (w/v) Sodium Azide
• Source: Rabbit
• Purity: Rabbit IgG F(ab)2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG, anti-Rabbit IgG F(ab)2 and anti-Rabbit Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
• Form: Lyophilized

Application Notes

• Application Dilute: ELISA: User Optimized, WB: User Optimized
• Application Notes: Biological Origin: Rabbit. Application Notes: Rabbit IgG F(ab)2 Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments

Images

SDS PAGE gel of commercially-purified rabbit Immunoglobulin G (rIgG) and of rIgG papain digestion products Fab and Fc. Lanes C-F contain products which have been reduced with b-mercaptoethanol. Lanes H-L contain non-reduced products. Each lane contains approximately 35 mg of the following products: Lanes C and H contain papain digested products, Lanes D, I and L contain Fc fragment obtained from protein A purification, Lanes E and J contain whole rIgG, Lanes F and K contain Fab fragment obtained from protein A purification, Lanes A, B and G contain gel markers. Whole rIgG (p/n orb2652747), rFc (p/n orb346310), rFab (p/n orb2652745), rF(ab)2 (p/n orb346311).

SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n orb346310). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n orb346313). Load: 1 µg for F(ab) and F(c), 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.