Rat Immunoglobulin G, Rat IgG whole molecule, Rat IgG purified protein
Rat IgG Antibody
Concentration:
10.0 mg/mL
Buffer:
0.01% (w/v) Sodium Azide
Source:
Rat
Purity:
Rat IgG was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat Serum.
Form:
Lyophilized
Application Dilute:
ELISA: User Optimized, FC: User Optimized, WB: User Optimized
Application Notes:
Biological Origin: Rat. Application Notes: Rat IgG whole molecule has been tested by SDS-Page and Western Blot and is suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins
SDS PAGE of Rat IgG Whole Molecule. Lane 1: Non-Reduced Rat IgG Whole Molecule. Lane 2: 5 µl Opal Prestained Marker. Lane 3: Reduced Rat IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-Reduced at 160 kDa/Observed at 245 kDa, Reduced at 55, 25 kDa. Non-reduced IgG migrates slightly higher.
SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated. Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule (p/n orb2652744). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated. Lane 3: Reduced Rat IgG Fab Fragment (p/n orb346340). Lane 4: Reduced Rat IgM Whole Molecule (p/n orb346341). Load: 1 µg of IgG, F(c), Fab, 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa, F(c) at 25 kDa, Fab at 25 kDa, IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.
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