0.01% (w/v) Sodium Azide. 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Source:
Rat
Purity:
Rat IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Rat IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum and anti-Rat IgM (µ chain specific). No reaction was observed against anti-Rat IgG F(c). Some light chain cross-reactivity will occur with anti-Rat IgG.
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Biological Origin: Rat. Application Notes: Rat IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments
SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated. Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule (p/n orb2652744). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated. Lane 3: Reduced Rat IgG Fab Fragment (p/n orb346340). Lane 4: Reduced Rat IgM Whole Molecule (p/n orb346341). Load: 1 µg of IgG, F(c), Fab, 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa, F(c) at 25 kDa, Fab at 25 kDa, IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.
SDS-Page of Rat IgM Whole Molecule. Lane 1: Rat IgM, Non-Reduced. Lane 2: Rat IgM, Reduced. Load: 1.0 µg per lane. Predicted/Observed size-Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 78 and 25 kDa.
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