0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Sheep
Purity:
Sheep IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Sheep IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep IgG and anti-Sheep Serum.
Form:
Lyophilized
Application Dilute:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Biological Origin: Sheep. Application Notes: Sheep IgG whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments
SDS PAGE Results of Sheep IgG Whole Molecule. Lane 1: Sheep IgG Reduced [5 µg]. Lane 2: Sheep IgG Reduced [1 µg]. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: Sheep IgG Non-Reduced [1 µg]. Lane 5: Sheep IgG Non-Reduced [5 µg]. 4-20% Gel, Coomassie stained.
SDS-Page of Sheep IgG Lane 1: Sheep IgG Non-Reduced Lane 2: Sheep IgG Reduced Load: 1.0 ug per lane Non-Reduced Predicted/Obsevered Size: 160 kDa/160 kDa Reduced Predicted/Obsevered Size: 28 and 55 kDa/28 and 55 kDa.
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