Armenian Hamster IgG isotype Control Antibody, Unconjugated
Biozol Catalog Number:
BYT-ORB346446
Supplier Catalog Number:
orb346446
Alternative Catalog Number:
BYT-ORB346446-1
Manufacturer:
Biorbyt
Category:
Proteine/Peptide
Application:
FC, SDS-PAGE
Conjugation:
Unconjugated
Alternative Names:
Armenian Hamster IgG isotype Control, A. Hamster IgG control, Control Protein, Isotype Protein
Armenian Hamster IgG isotype Control Antibody
Concentration:
1.0 mg/mL
Buffer:
0.01% (w/v) Sodium Azide
Source:
Armenian Hamster
Purity:
ARMENIAN HAMSTER IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Armenian Hamster IgG. Greatly diminished reactivity will occur against anti-Golden Syrian Hamster IgG.
Form:
Lyophilized
Application Dilute:
FC: 1:1000-1:5000
Application Notes:
Biological Origin: Armenian Hamster. Application Notes: ARMENIAN HAMSTER IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by user
Anti-CD154-facilitated alloengraftment is multilineage. Twenty mice from 2 representative experiments were phenotyped at 120 days after BMT for donor-host origin of CD4+ and CD8 + T cells, CD19 + B cells, and MAC-1 + myeloid cells. On the x-axis are shown the host and donor proportions of each of the lineages. ▪ indicates the proportion of each lineage of host origin, ▥, the proportion of each lineage that is of donor origin. On the y-axis is shown the percentage of PBLs of each lineage. Irrelevant hIgG-treated mice had no detectable donor chimerism and thus are composed entirely of host-type cells. Note that most CD4+ T cells, CD19 + B cells, and MAC-1 + myeloid cells in anti-CD154-treated mice are of donor origin. In contrast, most of the CD8 + T cells are of host origin. irrelevant hamster IgG (hIgG) (p/n orb346446).
SDS PAGE Results of Armenian Hamster IgG Whole Molecule Isotype Control. Lane 1: Armenian Hamster IgG Reduced [5.0 µg]. Lane 2: Armenian Hamster IgG Reduced [1.0 µg]. Lane 3: Lane 4: Armenian Hamster IgG Non-Reduced [1.0 µg]. Lane 5: Armenian Hamster IgG Non-Reduced [5.0 µg]. 4-20% Gel, Coomassie Stained.
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