Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Chinese Hamster
Purity:
The cells were grown in Hams F12 medium containing 2 mM L-Gln and 1.5 g/L sodium bicarbonate supplemented with 10% FBS (Fetal Bovine Serum). Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride,
Form:
Liquid
Application Dilute:
WB: User Optimized
Application Notes:
ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been
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