A431 Cell Nuclear Extract, Unconjugated

Product Overview

• Article Name: A431 Cell Nuclear Extract, Unconjugated
• Biozol Catalog Number: BYT-ORB348679
• Supplier Catalog Number: orb348679
• Alternative Catalog Number: BYT-ORB348679-200
• Manufacturer: Biorbyt
• Category: Sonstiges
• Application: WB
• Conjugation: Unconjugated
• Alternative Names: A431 Cell Nuclear Extract, A431 Nuclear Lysate, A431 Lysate Nuclear Extract

Product Description

A431 Cell Nuclear Extract

Product Properties

• Concentration: 1.0 mg/ml
• Buffer: None. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Source: Human
• Purity: The cells were grown in DMEM supplemented with 10% FBS (Fetal Bovine Serum). The lysate was prepared by first washing the cells in PBS. Washed cells were then incubated on ice in lysis buffer containing 10 mM HEPES, 60 mM KCl, 1.0 mM EDTA, 0.075% (v/v) N
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: WB: User Optimized
• Application Notes: ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5