HeLa Whole Cell Lysate Doxorubicin Stimulated, Unconjugated
Biozol Catalog Number:
BYT-ORB348681
Supplier Catalog Number:
orb348681
Alternative Catalog Number:
BYT-ORB348681-500
Manufacturer:
Biorbyt
Category:
Sonstiges
Application:
SDS-PAGE, WB
Conjugation:
Unconjugated
Alternative Names:
HeLa Whole Cell Lysate Doxorubicin Stimulated, HeLa WCL Doxorubicin Stimulated, HeLa Doxorubicin Stimulated Whole Cell Lysate, HeLa Doxorubicin Stimulated WCL
HeLa Whole Cell Lysate Doxorubicin Stimulated
Concentration:
1.0 mg/ml
Buffer:
Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Human
Purity:
The cells were grown in DMEM supplemented with 10% FBS (Fetal Bovine Serum). Cells were treated with 0.5 µg/ml Doxorubicin for 2 h. Cells were washed in PBS and incubated on ice in modified RIPA buffer containing 150 mM sodium chloride, 50 mM Tris Cl, pH
Form:
Liquid (sterile filtered)
Application Dilute:
WB: User Optimized
Application Notes:
ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been
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