HeLa Cell Nuclear Extract Nocodozole Stimulated, Unconjugated
Biozol Catalog Number:
BYT-ORB348686
Supplier Catalog Number:
orb348686
Alternative Catalog Number:
BYT-ORB348686-200
Manufacturer:
Biorbyt
Category:
Sonstiges
Application:
SDS-PAGE, WB
Conjugation:
Unconjugated
Alternative Names:
HeLa Cell Nuclear Extract Nocodazole Stimulated, HeLa Nocodazole Stimulated Nuclear Lysate, HeLa Nuclear Lysate Nocodazole Stimulated, HeLa Nocodazole Stimulated Nuclear Extract
HeLa Cell Nuclear Extract Nocodozole Stimulated
Concentration:
1.0 mg/ml
Buffer:
Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Human
Purity:
The cells were grown in DMEM supplemented with 10% FBS (Fetal Bovine Serum). Cells were treated with 0.2 µg/ml Nocodazole for 30 min. The lysate was prepared by first washing the cells in PBS. Washed cells were then incubated on ice in lysis buffer conta
Form:
Liquid (sterile filtered)
Application Dilute:
WB: User Optimized
Application Notes:
ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5
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