MCF-7 Cell Nuclear Extract, Unconjugated

Product Overview

• Article Name: MCF-7 Cell Nuclear Extract, Unconjugated
• Biozol Catalog Number: BYT-ORB348687
• Supplier Catalog Number: orb348687
• Alternative Catalog Number: BYT-ORB348687-200
• Manufacturer: Biorbyt
• Category: Sonstiges
• Application: WB
• Conjugation: Unconjugated
• Alternative Names: MCF-7 Cell Nuclear Extract, MCF7 Cell Nuclear Extract, MCF-7 Nuclear Lysate, MCF7 Lysate Nuclear Extract

Product Description

MCF-7 Cell Nuclear Extract

Product Properties

• Concentration: 1.0 mg/ml
• Buffer: Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Source: Human
• Purity: The cells were grown in EMEM supplemented with 10% FBS (Fetal Bovine Serum). Cells were washed in PBS and incubated on ice in modified RIPA buffer containing 150 mM sodium chloride, 50 mM Tris Cl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic ac
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: WB: User Optimized
• Application Notes: ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5