HeLa Cell Nuclear Extract TNFa Stimulated, Unconjugated
Biozol Catalog Number:
BYT-ORB348688
Supplier Catalog Number:
orb348688
Alternative Catalog Number:
BYT-ORB348688-200
Manufacturer:
Biorbyt
Category:
Sonstiges
Application:
WB
Conjugation:
Unconjugated
Alternative Names:
HeLa Cell Nuclear Extract TNFa Stimulated, HeLa Nuclear Lysate TNF alpha Stimulated, HeLa TNF alpha Stimulated Nuclear Lysate, HeLa Lysate Tumor Necrosis Factor Nuclear Extract
HeLa Cell Nuclear Extract TNFa Stimulated
Buffer:
Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Human
Purity:
The cells were grown in DMEM supplemented with 10% FBS (Fetal Bovine Serum). Cells were treated with 0.2 µg/ml TNFalpha for 30 min. The lysate was prepared by first washing the cells in PBS. Washed cells were then incubated on ice in lysis buffer containing
Form:
Liquid (sterile filtered)
Application Dilute:
WB: User Optimized
Application Notes:
ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5
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