Preservative: None. Stabilizer: None. 1X RIPA Buffer with HALT Protease and Phosphatase Inhibitors
Source:
Human
Purity:
Hep-G2 cells were grown in Eagles Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0%
Form:
Liquid (sterile filtered)
Application Dilute:
ChIP: User Optimized, IP: User Optimized, WB: User Optimized
Application Notes:
Multi-purpose Hep-G2 nuclear extracts are especially prepared as positive control for multiple assays including western blot, immunoprecipitation (IP), capture ELISA or other assays requiring native protein sample. For separation by SDS-PAGE and subseque
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