Protein A Peroxidase Conjugated, HRP

Product Overview

• Article Name: Protein A Peroxidase Conjugated, HRP
• Biozol Catalog Number: BYT-ORB348750
• Supplier Catalog Number: orb348750
• Alternative Catalog Number: BYT-ORB348750-1
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: DOT, ELISA, IHC, WB
• Conjugation: HRP
• Alternative Names: HRP, peroxidase, Horseradish peroxidase protein A

Product Description

Protein A Peroxidase Conjugated

Product Properties

• Concentration: 1.0 mg/mL
• UniProt: P38507
• Buffer: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: Protein A Peroxidase was prepared from chromatographically pure Protein A. Protein A Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.
• Form: Lyophilized

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000
• Application Notes: Application Notes: Protein A Peroxidase Conjugate has been tested by dot blot and is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110)

Images

Diagnostic accuracy of detecting IgG and IgG/M antibodies against natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) using HRP anti-human IgG (p/n orb347230), HRP anti-human IgM (p/n orb347297), and HRP Protein A. ELISA = enzyme-linked immunosorbent assay, NPV = negative predictive value, PPV = positive predictive value. Results are shown as percent, with confidence intervals in parentheses.

Dot Blot of PEROXIDASE Conjugated Protein A. Lane 1: Human IgG. Lane 2: Golden Syrian Hamster IgG. Lane 3: Sheep IgG. Load: 3-fold serial dilution starting at 200 ng. Primary Antibody: none. Secondary antibody: PEROXIDASE Conjugated Protein A at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS 30 min at RT.