Application Notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human IGFBP-7, His Tag (Cat. No. orb348791) immobilized on CM5 Chip can bind Human C1q R1, His Tag (Cat. No. orb257231) with an affinity constant of 16.7 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
SDS-PAGE analysis of Human IGFBP-7 protein
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