ULK1 Antibody (Biotin), Rabbit, Polyclonal

Product Overview

• Article Name: ULK1 Antibody (Biotin), Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB384045
• Supplier Catalog Number: orb384045
• Alternative Catalog Number: BYT-ORB384045-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide from the mid-protein of Human ULK1 (aa. 567-577)
• Conjugation: Biotin
• Alternative Names: Unc-51 like kinase 1 (C. elegans), Autophagy related protein 1 homolog, Autophagy-related protein 1 homolog, FLJ38455, Serine/threonine-protein kinase ULK1, Unc51.1, ATG1A, UNC51, C. elegans, homolog of, EC:2.7.11.1, Serine/threonine protein kinase Unc51.1, FLJ46475, ATG 1, hATG1, ATG1, Unc-51-like kinase 1, Serine/threonine protein kinase ULK1, ULK1, Unc 51 like kinase 1, KIAA0722, UNC 51, ULK 1, ULK1_HUMAN, UNC51, Unc 51 (C. elegans) like kinase 1, ATG1 autophagy related 1 homolog

Product Description

Rabbit polyclonal antibody against ULK1 conjugated to Biotin.

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 100 kDa
• NCBI: 003556
• UniProt: O75385
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Target: ULK1

Application Notes

• Application Dilute: WB (1:1000), ICC/IF (1:100)
• Application Notes: Application Notes: A 1:1000 dilution of SPC-640 was sufficient for detection of ULK1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody

Images

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ULK1 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain, DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK1 Antibody (D) Composite.

Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 112.6 kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa (15ug). Lane 3: Human 293Trap (15ug). Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 112.6 kDa.

Western blot analysis of Rat Brain cell lysates showing detection of ~112.6kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~112.6kDa.