HSP90 alpha Antibody (Biotin), IgG2a, Clone: [Hyb-K41009], Mouse, Monoclonal

Product Overview

• Article Name: HSP90 alpha Antibody (Biotin), IgG2a, Clone: [Hyb-K41009], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB396182
• Supplier Catalog Number: orb396182
• Alternative Catalog Number: BYT-ORB396182-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant human HSP90alpha, Specificity mapped to amino acids 604-731
• Conjugation: Biotin
• Alternative Names: HSP90AA1, HSP90-alpha, HSP90A, HSPCA, HSPC1, Heat shock protein HSP 90-alpha, Heat shock 90 kDa protein 1 alpha isoform, HSPCAL3, HSP86, HSP89A, HSP90Alpha

Product Description

Mouse monoclonal antibody against HSP90 alpha conjugated to Biotin.

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [Hyb-K41009]
• Molecular Weight: 90kDa
• Isotype: IgG2a
• NCBI: 001017963
• UniProt: P07900
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: HSP90 alpha

Application Notes

• Application Dilute: WB (1:1000), IHC (1:5000)
• Application Notes: Application Notes: 1 µg/ml of SMC-108 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Images

Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Western Blot analysis of Rat Lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells.