Sulfotyrosine Antibody (Biotin), IgG1, Clone: [12B11 Formerly sold as 7C5], Mouse, Monoclonal

Product Overview

• Article Name: Sulfotyrosine Antibody (Biotin), IgG1, Clone: [12B11 Formerly sold as 7C5], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB396795
• Supplier Catalog Number: orb396795
• Alternative Catalog Number: BYT-ORB396795-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC, WB
• Species Reactivity: All
• Immunogen: Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
• Conjugation: Biotin
• Alternative Names: Sulfotyrosine, Tyrosine sulfation, Sulfated Tyrosine, S-Tyrosine, sTYR, Sulphotyrosine, Tyrosine Sulphation, Sulphated Tyrosine

Product Description

Mouse monoclonal antibody against Sulfotyrosine conjugated to Biotin.

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [12B11 Formerly sold as 7C5]
• Isotype: IgG1
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: Sulfotyrosine

Application Notes

• Application Dilute: WB (1:1000), ICC/IF (1:50), ELISA (1:1000), IHC (1:25)
• Application Notes: Application Notes: A 1:1000 dilution of SMC-522 was sufficient for detection of Sulfotyrosine in 0.5 µg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody

Images

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 12B11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain, DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody. (D) Composite.

Immunohistochemistry analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 12B11. Tissue: kidney. Species: Human. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633, DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody (D) Composite.

Western Blot analysis of Sulfotyrosine-BSA Conjugate showing detection of 67 kDa Sulfotyrosine protein using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 12B11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Sulfotyrosine-BSA. Lane 3: Tyrosine-BSA. Lane 4: Phosphotyrosine-BSA. Lane 5: BSA. Load: 2 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.