BAP135, WBSCR6, General transcription factor II-I, GTFII-I, TFII-I, Bruton tyrosine kinase-associated protein 135, BAP-135, BTK-associated protein 135, SRF-Phox1-interacting protein, SPIN, Williams-Beuren syndrome chromosomal region 6 protein
The GTF2I Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide of human GTF2I .Purification: The antibody was purified by immunogen affinity chromatography.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Application Dilute:
WB: 1:500-2000
Western blot analysis of GTF2I expression in HEK293T (A) whole cell lysates. (Predicted band size: 30, 107, 110, 112 kD, Observed band size: 135 kD)
Immunofluorescent analysis of GTF2I staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
* VAT and and shipping costs not included. Errors and price changes excepted
