The NMDAR2A Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human NMDAR2A .Purification: The antibody was purified by immunogen affinity chromatography.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Application Dilute:
WB: 1:500-2000
Western blot analysis of NMDAR2A expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 144, 165 kD, Observed band size: 180 kD)
Immunofluorescent analysis of NMDAR2A staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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