Preservative: None. Stabilizer: None. See application note.
Source:
Mouse
Purity:
Mouse Transferrin was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis against the buffer stated above. Mouse Transferrin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Transferrin and anti-Mouse Serum.
Form:
Liquid (sterile filtered)
Target:
Trf
Application Dilute:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Biological Origin: Mouse. Application Notes: Mouse Transferrin is a suitable protein for use as a control reagent in SDS, Western Blotting, and ELISA experiments. For blotting, the expected molecular weight of Mouse Transferrin is approximately 80kDa. Mouse Transferrin, when unopened, is stable at 4 C, but for extended use should be aliquoted and stored at -20 C in its undiluted form. If dilutions of Mouse Transferrin are required, they should be prepared just prior to use and not retained
Fate of a preformed transferrin corona in vivo. A, activity of 59Fe and 125I (1-120 min) in blood, B and C, activity of 59Fe and 125I in organs 120 min after i.v. injection of 59Fe-SPIOs with a preformed protein corona of adsorbed (A, B right side) or covalently bound (A, B, left side) 125I-mouse-transferrin. mouse transferrin (p/n orb420186).
SDS-Page of Mouse Transferrin Lane 1: Mouse Transferrin Load: 1.0 µg per lane Predicted/Observed size: 80 kDa, 80 kDa.
The addition of C3 or fibronectin to QF enhanced hepatic transgene expression. Lipoplex was incubated with each component for 5 min before intraportal injection. QF, QAE column flow through fraction, C3, complement component C3, Fet, fetuin, AF, asialofetuin, FN, fibronectin, Tf, transferrin. Each bar represents the mean SE of at least seven experiments. *p < 0.05, **p < 0.01 versus QF group. Mouse transferrin (Tf) (p/n orb420186).
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