PARP1 (N-term ZF1) Antibody, Unconjugated, Rabbit, Polyclonal Preis auf Anfrage

Product Overview

• Article Name: PARP1 (N-term ZF1) Antibody, Unconjugated, Rabbit, Polyclonal Preis auf Anfrage
• Biozol Catalog Number: BYT-ORB420276
• Supplier Catalog Number: orb420276
• Alternative Catalog Number: BYT-ORB420276-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: 2D-PAGE, IHC, WB
• Species Reactivity: Human
• Immunogen: PARP1 (N-term ZF1) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with n-terminus region of human PARP1 zinc finger domain recombinant protein.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-PARP1 Antibody, Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, PPOL

Product Description

PARP1 antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• UniProt: P09874
• Buffer: 0.01% (w/v) Sodium Azide
• Purity: PARP1 (N-term ZF1) was purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human PARP1 protein. No cross reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined.
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: IHC: 1:100, WB: 1:1000
• Application Notes: Application Notes: Anti-PARP1 (N-term ZF1) antibody has been validated by western blotting, IHC, and nanoimmunoassay (NIA). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 113 kDa in size corresponding to PARP-1 by western blotting in the appropriate cell lysate or extract

Images

Immunohistochemistry with anti-PARP-1 antibody showing nuclear positivity in human lung tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-PARP-1 antibody orb420276 at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 33-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.

OVCAR-8 Wild Type Lysate was separated on 2D SDS-PAGE and blotted on PVDF to analyze immunocoverage of PARP1 antibody specific for the zinc finger 1 domain of PARP1. Primary Antibody: Anti-PARP1 (n-term) antibody 1:200 overnight at 4C. Secondary Antibody: Goat anti-rabbit Peroxidase (orb347654) at 1:2000 at RT for 30 min. Blocking Buffer: BlockOut (p/n orb348644) for 30 min at RT. Predicted/observed: ~110 kDa and pI 9.7.

Peggy Sue(TM) Size Separation Electropherogram of OVCAR-8 lysates in no-salt buffer and detected with Anti-PARP1 (N-term ZF1). UV immobilization time: 250 seconds. Protein concentration: 577 µg/ml, 120 s UV immobilization. Primary antibody concentration: 20 µg/ml. Primary antibody incubation time: 180 min. Exposure time: multi-image analysis exposure. Predicted/observed: ~116 kDa.

Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 (N-term ZF1) Antibody. Lane 1: OVCAR8 Wild Type lysate. Lane 2: OVCAR8 PARP1 KO lysate. Load: 5 µg per lane. Primary antibody: PARP1 (N-term ZF1) antibody at 1 µg/ml for overnight at 4C. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 overnight at 4C. Predicted/Observed size: 113 kDa for endogenous PARP1. Other band(s): none.

Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 Antibodies. Lane 1: OVCAR8 Wild Type lysate. Lane 2: OVCAR8 PARP1 KO lysate. Load 5 µg per lane. Primary Antibody: Blot A: Anti-PARP1- n term (p/n orb420276), Blot B: Anti-PARP1- internal (p/n orb344711) at 1 µg/ml for overnight at 4C. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 overnight at 4C. Predicted/Observed size: 113 kDa for endogenous PARP1. Other band(s): nonspecific ~ 40 kDa in PARP1-AD only.

Western Blot of Rabbit anti-PARP1 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate (p/n orb348669). Lane 2: HeLa Lysate (p/n orb348668). Lane 3: MCF-7 Lysate (p/n orb348664). Lane 4: Jurkat Lysate. Lane 5: A431 Lysate (p/n orb348665). Lane 6: A549 Lysate (p/n orb348675). Lane 7: LNCap Lysate (p/n orb348694). Lane 8: MOLT-4 Lysate (p/n orb348696). Lane 9: Ramos Lysate. Lane 10: Raji Lysate (p/n orb348672). Lane 11: A-172 Lysate (p/n orb348708). Lane 12: NIH/3T3 Lysate (p/n orb348714). Load: 35 µg per lane. Primary antibody: PARP1 antibody at 1 ug/ml overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:30000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 113 kDa for PARP1.

Western Blot of Rabbit anti-PARP1 N-term Antibody. Lane 1: Opal Pre-stained ladder. Lane 2: OVCAR-8 Wild Type. Lane 3: PARP1-KO. Lane 4: PARP2-KO. Lane 5: PARP3-KO. Lane 6: PARP4-KO Lane 7: PARP5a-KO. Lane 8: PARP5b-KO. Lane 9: PARP6-KO. Lane 10: PARP7-KO. Lane 11: PARP8-KO. Lane 12: PARP9-KO. Lane 13: PARP10-KO. Lane 14: PARP12-KO. Lane 15: PARP13-KO. Lane 16: PARP14-KO. Lane 17: PARP16-KO. Load: 5.0 µg per lane. Primary antibody: PARP1 n-term antibody at 1 ug/ml overnight at 4C. Secondary antibody: Goat anti-rabbit Peroxidase secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Blocking Buffer: orb348644 for 30 min at RT. Predicted/Observed size: ~113 kDa for PARP1.

Western Blot o