Phosphotyrosine Antibody Biotin Conjugated, Rabbit, Polyclonal Preis auf Anfrage
Biozol Catalog Number:
BYT-ORB420481
Supplier Catalog Number:
orb420481
Alternative Catalog Number:
BYT-ORB420481-100
Manufacturer:
Biorbyt
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, IHC, IP, WB
Species Reactivity:
Other
Immunogen:
Anti-PhosphoTyrosine was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.
Conjugation:
Biotin
Alternative Names:
PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit
Phosphotyrosine antibody (Biotin)
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Buffer:
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 50% (v/v) Glycerol, Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-PhosphoTyrosine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose followed by conjugation to biotin and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin. Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphothreonine or phosphoserine.
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: 1:5,000, IHC: 10µg/mL, WB: 1:1,000
Application Notes:
Application Notes: Anti-PhosphoTyrosine antibody is tested for use in ELISA, immunohistochemistry, IP, and western blotting assays. The antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA and flow cytometry
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