This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with methylated lysine conjugated KLH.
Conjugation:
Biotin
Alternative Names:
Dimethylysine antibody, Methyl lysine antibody, N epsilon dimethyl lysine antibody, N epsilon mono methyllysine antibody, N epsilon trimethyl lysine antibody, N(epsilon) trimethyl lysine antibody, Trimethyl lysine antibody
Lysine Methylated antibody (Biotin)
Clonality:
Polyclonal
Concentration:
1 mg/mL
Buffer:
Preservative: None. Stabilizer: 50% (v/v) Glycerol, Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using methylated lysine peptide coupled to agarose followed by conjugation to biotin and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin. Reactivity is specific for methylated lysine.
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: 1:5,000, WB: 1:1,000
Application Notes:
Application Notes: This affinity-purified antibody is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with methylated lysine residues. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry
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