Mouse GM CSF Protein

Product Overview

• Article Name: Mouse GM CSF Protein
• Biozol Catalog Number: BYT-ORB425784
• Supplier Catalog Number: orb425784
• Alternative Catalog Number: BYT-ORB425784-1, BYT-ORB425784-20, BYT-ORB425784-5
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, CSF2, GMCSF

Product Description

Recombinant of mouse GM CSF protein

Product Properties

• Buffer: GM-CSF Mouse was lyophilized with no additives.
• Purity: Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg

Application Notes

• Application Notes: Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg. Application Notes: Protein content: GM-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard