Phospho-Bad (Ser118) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-Bad (Ser118) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB4670
• Supplier Catalog Number: orb4670
• Alternative Catalog Number: BYT-ORB4670-100,BYT-ORB4670-200,BYT-ORB4670-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human BAD around the phosphorylation site of Ser118 RM(p-S)DE
• Conjugation: Unconjugated
• Alternative Names: Bad(phospho S118), Bad(phospho Ser118), p-Bad(S118), p-Bad(Ser118), p-Bad(phospho Ser118), BBC 2, BBC2, BBC6, Bcl 2 Antagonist of Cell Death, Bcl 2 Binding Component 6, BCL X/BCL 2 Binding Protein, BCL X Binding Protein, Bcl XL/Bcl 2 Associated Death Promoter, Bcl-2-like protein 8, Bcl2 antagonist of cell death, BCL2 antagonist of cell death protein, BCL2 associated agonist of cell death, Bcl2 Associated Death Promoter, BCL2 binding component 6, BCL2 binding protein, Bcl2 Like 8 Protein, Bcl2-L-8, BCL2L8, BclXL, Proapoptotic BH3 Only Protein, BAD_HUMAN, Bcl-2-binding component 6.

Product Description

Phospho-Bad (Ser118) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 22 kDa
• UniProt: Q92934
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: BAD

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Application Notes: Modification: Phosphorylated

Images

Blank control (Black line): Jurkat (Black). Primary Antibody (green line): Rabbit Anti-Bad (Ser118) antibody (orb4670), dilution: 3 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-Phospho-Bad (Ser118) antibody (orb4670), dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Bad (Ser118)) Polyclonal Antibody, Unconjugated (orb4670) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Bad (Ser118)) Polyclonal Antibody, Unconjugated (orb4670) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Bad (Ser118)) Polyclonal Antibody, Unconjugated (orb4670) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, Hela KO Bad (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Bad (Ser118) (orb4670) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Bad (Ser118) (orb4670) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Sample: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-Phospho-Bad (Ser118) (orb4670) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

IHC-P analysis of Rat brain using Phospho-Bad Ser118 Polyclonal Antibody at 1:400 dilution.