Phospho-BRAF (Thr401) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-BRAF (Thr401) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB4720
• Supplier Catalog Number: orb4720
• Alternative Catalog Number: BYT-ORB4720-50,BYT-ORB4720-100,BYT-ORB4720-200
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human B-Raf around the phosphorylation site of Thr401 SA(p-T)PP
• Conjugation: Unconjugated
• Alternative Names: BRAF (p-T401), p-BRAF, phospho-BRAF, B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1, 9930012E13Rik, Braf-2, Braf2, C230098H17, D6Ertd631e, BRAF_HUMAN, BRAF, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, 2.7.11.1, BRAF_MOUSE,

Product Description

Phospho-BRAF (Thr401) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 94 kDa
• UniProt: P15056
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: BRAF

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-BRAF (Thr401)) Polyclonal Antibody, Unconjugated (orb4720) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-BRAF (Thr401)) Polyclonal Antibody, Unconjugated (orb4720) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Thr401)) Polyclonal Antibody, Unconjugated (orb4720) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Thr401)) Polyclonal Antibody, Unconjugated (orb4720) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Thr401)) Polyclonal Antibody, Unconjugated (orb4720) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Thr401)) Polyclonal Antibody, Unconjugated (orb4720) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-B-Raf (Thr401)) Polyclonal Antibody, Unconjugated (orb4720) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P analysis of mouse brain using phospho-B-Raf Thr401 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of rat placenta using phospho-B-Raf Thr401 Polyclonal Antibody at 1:200 dilution.