Histone H3 (mono methyl K79) Mouse Monoclonal Antibody, IgG1, Clone: [3D6], Unconjugated

Product Overview

• Article Name: Histone H3 (mono methyl K79) Mouse Monoclonal Antibody, IgG1, Clone: [3D6], Unconjugated
• Biozol Catalog Number: BYT-ORB499568
• Supplier Catalog Number: orb499568
• Alternative Catalog Number: BYT-ORB499568-50,BYT-ORB499568-100,BYT-ORB499568-200
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of mono methyl K79 DF(mono methyl K)TD
• Conjugation: Unconjugated
• Alternative Names: H3C1 | Histone H3 (me1-K79), me-Histone H3, methyl-Histone H3, H3K79me1, me-H3, H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, H3/b, H3C1, H3FB, HIST1H3D, H3.1, H3/c, H3FC, HIST1H3C, H3/d, H3FD, HIST1H3E, H3.f, H3/f, H3FF, HIST1H3I, H3/h, H3FH, HIST1H3G, H3/j, H3FJ, HIST1H3J, H3/k, H3F1K, H3FK, HIST1H3H, H3/l, H3FL, HIST1H3B, H3/i, H3FI, HIST1H3F, H3.2-221, H3c13, H3c14, H3c15, Hist1, H3-143, H3-b, H3-53, H3-f, H3-291, H3-H, H3.1-221, H3 histone, Hist1h2ail, Hist1h2ail1, Hist2h3c, Hist3h3, H31_HUMAN, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FC HIST1H3C, H33_HUMAN, H3-3A, H3-3B, H3.3A, H3F3, H3F3A, H3.3B, H3F3B, H31T_HUMAN, H3-4, H3/t, H3t, H3/g, Histone H3.4, H3FT, H3C_HUMAN, H3-5, Histone H3.5, H3F3C, H32_HUMAN, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o, HIST2H3A, H3F2, H3FM, HIST2H3D, H31_MOUSE, H3a, H3g, H3.1-291, H3h, H3.1-I, H3i, H32_MOUSE, H3-clustered histone 2, H3-clustered histone 3, H3-clustered histone 4, H3-clustered histone 6, H3-clustered histone 7, H3.2, H3b, H3f, H3.2-616, Hist2h3b, H3.2-615, Hist2h3c1, Hist2h3ca1, H3.2-614, Hist2h3c2, Hist2h3ca2, H31_RAT,

Product Description

Histone H3 (mono methyl K79) Mouse Monoclonal Antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [3D6]
• Molecular Weight: 15 kDa
• Isotype: IgG1
• UniProt: P68431
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: H3C1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:1000-5000, IHC-F=1:1000-5000, IF=1:1000-5000

Images

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3 (mono methyl K79)) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3 (mono methyl K79)) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3 (mono methyl K79)) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3 (mono methyl K79)) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3 (mono methyl K79)) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Monoclonal Antibody, Unconjugated (ascites of orb499568 3D6) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Monoclonal Antibody, Unconjugated (ascites of orb499568 3D6) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Monoclonal Antibody, Unconjugated (ascites of orb499568 3D6) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

IHC-P analysis of rat kidney using Histone H3 Monoclonal Antibody at 1:2000 dilution.