GPR49/LGR5 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: GPR49/LGR5 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB499632
• Supplier Catalog Number: orb499632
• Alternative Catalog Number: BYT-ORB499632-50,BYT-ORB499632-100,BYT-ORB499632-200
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human GPR49/LGR5 (331-430/907aa)
• Conjugation: Unconjugated
• Alternative Names: FEX, GPR49, GPR67, GRP49, HG38, LGR5_HUMAN, LGR5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5_MOUSE, Orphan G-protein coupled receptor FEX,

Product Description

GPR49/LGR5 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 98 kDa
• UniProt: O75473
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: LGR5

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (orb499632) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GPR49 LGR5) Polyclonal Antibody, Unconjugated (orb499632) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GPR49 LGR5) Polyclonal Antibody, Unconjugated (orb499632) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HepG2 (Human) Cell Lysate at 40 ug, Primary: Anti-LGR5 at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 98 kD, Observed band size: 115 kD.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 1: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-GPR49/LGR5 (orb499632) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kD, Observed band size: 105 kD.

IHC-P analysis of mouse pancreas using GPR49 LGR5 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of rat pancreas using GPR49 LGR5 Polyclonal Antibody at 1:200 dilution.