Beta tubulin Mouse Monoclonal Antibody (Loading Control), IgG1, Clone: [7G3], Unconjugated

Product Overview

• Article Name: Beta tubulin Mouse Monoclonal Antibody (Loading Control), IgG1, Clone: [7G3], Unconjugated
• Biozol Catalog Number: BYT-ORB499644
• Supplier Catalog Number: orb499644
• Alternative Catalog Number: BYT-ORB499644-100,BYT-ORB499644-200,BYT-ORB499644-500,BYT-ORB499644-1
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Beta tubulin
• Conjugation: Unconjugated
• Alternative Names: CDCBM6, CSCSC1, M40, OK/SW-cl.56, TUBB1, TUBB5, B130022C14Rik, M(beta)5, Tubb, TBB5_HUMAN, Tubulin beta-5 chain, TBB5_MOUSE, TBB5_RAT,

Product Description

Beta tubulin Mouse Monoclonal Antibody (Loading Control)

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [7G3]
• Molecular Weight: 50 kDa
• Isotype: IgG1
• UniProt: P07437
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: TUBB

Application Notes

• Application Dilute: WB=1:10000-50000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

Images

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Beta tubulin) monoclonal Antibody, Unconjugated (orb499644) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Beta tubulin (Loading Control)) Monoclonal Antibody, Unconjugated (ascites of orb499644 7G3) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Beta tubulin (Loading Control)) Monoclonal Antibody, Unconjugated (orb499644) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Beta tubulin (orb499644) at 1/1000~20000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.

Sample: Hela (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, HL60 (Human) Cell Lysate at 30 ug, SH-SY5Y (Human) Cell Lysate at 30 ug, Cerebellum (Mouse) Lysate at 40 ug, Cerebellum (Rat) Lysate at 40 ug, Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Beta tubulin (orb499644) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Hela (Human) Lysate at 40 ug, Primary: Anti-Beta tubulin (orb499644) at 1/2000~1/20000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.

IHC-P analysis of Mouse brain using Beta tubulin Monoclonal Antibody at 1:400 dilution.