Phospho-SMAD7 (Ser249) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-SMAD7 (Ser249) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB500554
• Supplier Catalog Number: orb500554
• Alternative Catalog Number: BYT-ORB500554-50,BYT-ORB500554-100,BYT-ORB500554-200
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human SMAD7 around the phosphorylation site of Ser249 SD(p-S)QL
• Conjugation: Unconjugated
• Alternative Names: SMAD7 (p-S249), p-SMAD7, phospho-SMAD7, Madh7, SMAD7_HUMAN, SMAD7, MAD homolog 7, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8 (MAD homolog 8 | Mothers against DPP homolog 8), SMAD family member 7 (SMAD 7 | Smad7 | hSMAD7), MADH8, SMAD7_MOUSE, SMAD family member 7 (SMAD 7 | Smad7), SMAD7_RAT,

Product Description

Phospho-SMAD7 (Ser249) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 46 kDa
• UniProt: O15105
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: SMAD7

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-SMAD7 (Ser249)) Polyclonal Antibody, Unconjugated (orb500554) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-SMAD7 (Ser249)) Polyclonal Antibody, Unconjugated (orb500554) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-SMAD7 (Ser249)) Polyclonal Antibody, Unconjugated (orb500554) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-SMAD7 (Ser249)) Polyclonal Antibody, Unconjugated (orb500554) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Primary: Anti-Phospho-SMAD7 (Ser249) (orb500554) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 50 kDa.