CD200 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: CD200 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB500587
• Supplier Catalog Number: orb500587
• Alternative Catalog Number: BYT-ORB500587-50,BYT-ORB500587-100,BYT-ORB500587-200
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human CD200 (41-140/278aa)
• Conjugation: Unconjugated
• Alternative Names: MOX1, MOX2, MRC, OX-2, OX2G_HUMAN, CD200,

Product Description

CD200 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 40-50 kDa
• UniProt: P41217
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: CD200

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (orb500587) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (orb500587) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (orb500587) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CD200 Polyclonal Antibody, Unconjugated (orb500587) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human rectal carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CD200 Polyclonal Antibody, Unconjugated (orb500587) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

IHC-P analysis of human lung carcinoma using Anti-CD200 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of rat ovary using CD200 Polyclonal Antibody at 1:200 dilution.