S100B Mouse Monoclonal Antibody, Clone: [1C6], Unconjugated

Product Overview

• Article Name: S100B Mouse Monoclonal Antibody, Clone: [1C6], Unconjugated
• Biozol Catalog Number: BYT-ORB500644
• Supplier Catalog Number: orb500644
• Alternative Catalog Number: BYT-ORB500644-50,BYT-ORB500644-100,BYT-ORB500644-200
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant human S100B protein (2-92/92aa (N-6x His-Tag)aa)
• Conjugation: Unconjugated
• Alternative Names: NEF, S100, S100-B, S100beta, S100B_HUMAN, S100B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B,

Product Description

S100B Mouse Monoclonal Antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [1C6]
• Molecular Weight: 10 kDa
• UniProt: P04271
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: S100B

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

Images

(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human glioblastoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of orb500644 3C2) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Sample: Lane 1: Cerebral cortex (Rat) Lysate at 40 ug, Lane 2: Spinal cord (Mouse) Lysate at 40 ug, Lane 3: Spinal cord (Rat) Lysate at 40 ug, Lane 4: Cerebellum (Mouse) Lysate at 40 ug, Lane 5: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-S100B (orb500644) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 11 kD, Observed band size: 9 kD.

IHC-P analysis of mouse spinal cord using S100B Monoclonal Antibody at 1:2000 dilution.

IHC-P analysis of rat spinal cord using S100B Monoclonal Antibody at 1:2000 dilution.