Phospho-Tau Protein (Thr181) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-Tau Protein (Thr181) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB500682
• Supplier Catalog Number: orb500682
• Alternative Catalog Number: BYT-ORB500682-100,BYT-ORB500682-200,BYT-ORB500682-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Thr181 PK(p-T)PP
• Conjugation: Unconjugated
• Alternative Names: MAPT | Tau (p-T181), p-Tau, phospho-Tau, tau, DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, Tau-PHF6, tau-40, Mtapt, PHF-tau, MAPT_0N4R, MAPT_1N4R, MAPT_2N4R, RNPTAU, pTau, TAU_BOVIN, MAPT, Neurofibrillary tangle protein, Paired helical filament-tau (PHF-tau), TAU_HUMAN, TAU_MOUSE, TAU_RAT,

Product Description

Phospho-Tau Protein (Thr181) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 52/79 kDa
• UniProt: P10636
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: MAPT

Application Notes

• Application Dilute: WB=1:500-2000
• Application Notes: Modification: Phosphorylated

Images

Immunohistochemical staining of human brain glioma using Tau Protein (phospho-Thr181) antibody.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Tau Protein (Thr181)) Polyclonal Antibody, Unconjugated (orb500682) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Tau Protein (Thr181) (orb500682) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Sample: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Tau Protein (Thr181) (orb500682) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Sample: Lane 1: Human Tau-F Protein at 500 ng, Primary: Rabbit Anti-phospho-Tau Protein Antibody at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45.8kD, Observed band size: kD.

Western blot analysis of Hela(Human) Cell Lysate using Tau Protein (phospho-Thr181) antibody.

Western blot analysis of K562(Human) Cell Lysate using Tau Prote