Beta I Tubulin Mouse Monoclonal Antibody (Loading Control), IgG2a, Clone: [5F7], Unconjugated

Product Overview

• Article Name: Beta I Tubulin Mouse Monoclonal Antibody (Loading Control), IgG2a, Clone: [5F7], Unconjugated
• Biozol Catalog Number: BYT-ORB500698
• Supplier Catalog Number: orb500698
• Alternative Catalog Number: BYT-ORB500698-50,BYT-ORB500698-100,BYT-ORB500698-200
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human beta I Tubulin
• Conjugation: Unconjugated
• Alternative Names: MACTHC1, 2810484G07Rik, M(beta)1, TBB1_CHICK, Beta-tubulin class-I, TBB1_HUMAN, TUBB1, TBB1_MOUSE,

Product Description

Beta I Tubulin Mouse Monoclonal Antibody (Loading Control)

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [5F7]
• Molecular Weight: 50 kDa
• Isotype: IgG2a
• UniProt: Q9H4B7
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: TUBB1

Application Notes

• Application Dilute: WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-200, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (ascites of orb500698) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (orb500698 5F7) at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (ascites of orb500698) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (orb500698) at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, Cerebellum (Mouse) Lysate at 40 ug, Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-beta I Tubulin (orb500698) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-beta I Tubulin (orb500698) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Tissue/Cell: HeLa cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (orb500698) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Western blot analysis using Anti-beta I Tubulin antibody at 1:1000 dilution.

Western blot:Cerebrum (Mouse),Cerebrum (Rat),Primary:Anti-beta I Tubulin at 1:1000 dilution.