HIF-1 Alpha Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: HIF-1 Alpha Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB500743
• Supplier Catalog Number: orb500743
• Alternative Catalog Number: BYT-ORB500743-100,BYT-ORB500743-200,BYT-ORB500743-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, WB
• Species Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from human HIF-1 Alpha (661-760/826aa)
• Conjugation: Unconjugated
• Alternative Names: ARNT interacting protein, ARNT-interacting protein, Basic helix loop helix PAS protein MOP1, Basic-helix-loop-helix-PAS protein MOP1, bHLHe78, Class E basic helix-loop-helix protein 78, HIF 1A, HIF 1alpha, HIF-1-alpha, HIF1 A, HIF1 alpha, HIF1, HIF1-alpha, HIF1A, HIF1A_HUMAN, Hypoxia inducible factor 1 alpha, Hypoxia inducible factor 1 alpha isoform I.3, Hypoxia inducible factor 1 alpha subunit, Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor, Hypoxia inducible factor 1, alpha subunit(basic helix loop helix transcription factor), Hypoxia inducible factor1alpha, Hypoxia-inducible factor 1-alpha, Hypoxia-inducible factor-1a, Member of PAS protein 1, Member of PAS superfamily 1, Member of the PAS Superfamily 1, MOP 1, MOP1, PAS domain-containing protein 8, PASD 8, PASD8.

Product Description

HIF-1 Alpha Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 92 kDa
• UniProt: Q16665
• Buffer: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Form: Liquid
• Target: HIF1A

Application Notes

• Application Dilute: WB=1:1000-5000, Flow-Cyt=1µg/Test

Images

Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-HIF-1 Alpha antibody (orb500743), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (orb500743) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (orb500743) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (orb500743) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (orb500743) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Lane 4: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-HIF-1 Alpha (orb500743) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 92 kD, Observed band size: 120 kD.

Sample: Lane 1: Recombinant human HIF-1 Alpha protein, N-Trx-His, Primary: Anti-HIF-1 Alpha (orb500743) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 92 kDa, Observed band size: 52 kDa.

Tissue/Cell: U-87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, Unconjugated (orb500743) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of Rat kidney using HIF-1 Alpha Polyclonal Antibody at 1:200 dilution.