HSP90 alpha Mouse Monoclonal Antibody, Clone: [3H7], Unconjugated

Product Overview

• Article Name: HSP90 alpha Mouse Monoclonal Antibody, Clone: [3H7], Unconjugated
• Biozol Catalog Number: BYT-ORB500766
• Supplier Catalog Number: orb500766
• Alternative Catalog Number: BYT-ORB500766-50,BYT-ORB500766-100,BYT-ORB500766-200
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant human HSP90 alpha Protein (549-692/732aa)
• Conjugation: Unconjugated
• Alternative Names: EL52, HEL-S-65p, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp103, Hsp89, Hsp90, LAP-2, LAP2, 86kDa, 89kDa, Hsp86-1, hsp4, HS90A_HUMAN, HSP90AA1, Heat shock 86 kDa (HSP 86 | HSP86), Heat shock protein family C member 1, Lipopolysaccharide-associated protein 2 (LAP-2 | LPS-associated protein 2), Renal carcinoma antigen NY-REN-38, 3.6.4.10, HS90A_MOUSE, Tumor-specific transplantation 86 kDa antigen (TSTA), HS90A_RAT,

Product Description

HSP90 alpha Mouse Monoclonal Antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [3H7]
• Molecular Weight: 81 kDa
• UniProt: P07900
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: HSP90AA1

Application Notes

• Application Dilute: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (orb500766) at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (ascites of orb500766 3H7) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (orb500766) at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 40 ug, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

Sample: Hela Cell (Human) Lysate at 40 ug, NIH/3T3 Cell (Mouse) Lysate at 40 ug, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 98 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Placenta tissue lysates, Lane 4: Rat Placenta tissue lysates, Lane 5: Human U251 cell lysates, Lane 6: Human Molt-4 cell lysates, Lane 7: Human HL60 cell lysates, Lane 8: Human A549 cell lysates, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

Western blot:Hela