NeuN Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: NeuN Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB500816
• Supplier Catalog Number: orb500816
• Alternative Catalog Number: BYT-ORB500816-100,BYT-ORB500816-200,BYT-ORB500816-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human NeuN (51-150/312aa)
• Conjugation: Unconjugated
• Alternative Names: RFOX3_HUMAN, RNA binding protein fox-1 homolog 3, RBFOX3, Fox-1 homolog C, Neuronal nuclei antigen(NeuN antigen), FOX3, FOX-3, HRNBP3,

Product Description

NeuN Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 34 kDa
• UniProt: A6NFN3
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: RBFOX3

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NeuN) Polyclonal Antibody, Unconjugated (orb500816) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-NeuN (orb500816) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46/50 kD, Observed band size: 46/50 kD.

Western blot:Lane 1:Cerebrum (Mouse),Lane 2:Cerebellum (Mouse),Primary:Anti-NeuN at 1:1000 dilution.