GPR30 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: GPR30 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB500824
• Supplier Catalog Number: orb500824
• Alternative Catalog Number: BYT-ORB500824-100,BYT-ORB500824-200,BYT-ORB500824-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human GPR30 (251-350/375aa)
• Conjugation: Unconjugated
• Alternative Names: G Protein Coupled Receptor 30, G-protein coupled receptor 30, G-protein coupled estrogen receptor 1, Membrane estrogen receptor, mER, Chemokine receptor-like 2, IL8-related receptor DRY12, Flow-induced endothelial G-protein coupled receptor 1, FEG-1, Lymphocyte-derived G-protein coupled receptor, LYGPR, GPCR-BR, CEPR, CMKRL2, DRY12, GPER.

Product Description

GPR30 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 42 kDa
• UniProt: Q99527
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: GPER1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test

Images

Blank control (blue line): A431 cells (fixed with 70% methanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 20 min at -20C). Primary Antibody (green line): Rabbit Anti-GPR30 antibody (orb500824), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated (orb500824) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated (orb500824) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GPR30) Polyclonal Antibody, Unconjugated (orb500824) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Rat) Lysate at 40 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 5: MCF-7 (Human) Cell Lysate at 30 ug, Lane 6: Heart (Mouse) Lysate at 40 ug, Lane 7: Heart (Rat) Lysate at 40 ug, Primary: Anti-GPR30 (orb500824) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.

Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (GPR30) polyclonal Antibody, Unconjugated (orb500824) 1:100, 90 minutes at 37C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of rat brain using GPR30 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of mouse brain using GPR30 Polyclonal Antibody at 1:200 dilution.