INOS Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: INOS Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB501000
• Supplier Catalog Number: orb501000
• Alternative Catalog Number: BYT-ORB501000-100,BYT-ORB501000-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human NOS-2 (101-200/1153aa)
• Conjugation: Unconjugated
• Alternative Names: i NOS, Nitric Oxide Synthase, Inducible, HEP NOS, Hepatocyte NOS, HEPNOS, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, INOS, Inosl, MAC NOS, Macrophage NOS, Nitric oxide synthase 2 inducible macrophage, Nitric oxide synthase 2A(inducible hepatocytes), Nitric oxide synthase inducible, NOS 2, NOS 2A, NOS, Nos II, NOS type II, Nos2, NOS2A, NOS2_HUMAN.

Product Description

INOS Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 130 kDa
• Pubmed: 40833917
• UniProt: P35228
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: NOS2

Application Notes

• Application Dilute: WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000

Images

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (inos) Polyclonal Antibody, Unconjugated (orb501000) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (orb501000) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Raw264.7 tissue lysates, Lane 2: Human Jurkat cell lysates, Primary: Anti-iNOS (orb501000) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kDa, Observed band size: 140 kDa.

Sample: U251 Cell Lysate at 40 ug, Primary: Anti-iNOS (orb501000) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 130kD, Observed band size: 130kD.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated (orb501000) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated (orb501000) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Western blot:U251 Cell,Primary:Anti-iNOSat 1:300 dilution.

Western blot:Lane 1:Mouse Raw264.7,Lane 2:Human Jurkat cell,Primary:Anti-iNOS at 1:1000 dilution.