Phospho-AKT (Ser473) Mouse Monoclonal Antibody, Clone: [9H11], Unconjugated

Product Overview

• Article Name: Phospho-AKT (Ser473) Mouse Monoclonal Antibody, Clone: [9H11], Unconjugated
• Biozol Catalog Number: BYT-ORB506119
• Supplier Catalog Number: orb506119
• Alternative Catalog Number: BYT-ORB506119-100,BYT-ORB506119-200,BYT-ORB506119-50
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human AKT around the phosphorylation site of Ser473 QF(p-S)YS
• Conjugation: Unconjugated
• Alternative Names: Akt(phospho-Ser473), AKT(phospho-S473), AKT(phospho Ser473), p-AKT(Ser473), AKT 1, AKT, AKT1, AKT-1, AKT1_HUMAN, C AKT, cAKT, MGC9965, MGC99656, Oncogene AKT1, PKB, PKB alpha, PKB-ALPHA, PRKBA, Protein Kinase B alpha, Protein kinase B, Proto-oncogene c-Akt, RAC alpha, RAC alpha serine/threonine protein kinase, RAC, RAC PK alpha, Rac protein kinase alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, v akt murine thymoma viral oncogene homolog 1, vAKT Murine Thymoma Viral Oncogene Homolog 1.

Product Description

Phospho-AKT (Ser473) Mouse Monoclonal Antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [9H11]
• Molecular Weight: 56 kDa
• UniProt: P31749
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: AKT1

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:500-1000, IF=1:500-1000
• Application Notes: Modification: Phosphorylated

Images

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (ascites of orb506119 9H11) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (orb506119) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human skin carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (ascites of orb506119 9H11) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (ascites of orb506119 9H11) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (orb506119) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (orb506119) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

IHC-P analysis of mouse brain using phospho-AKT Ser473 Monoclonal Antibody at 1:200 dilution.

IHC-P analysis of rat brain using phospho-AKT Ser473 Monoclonal Antibody at 1:200 dilution.