Phospho-E2F1 (Ser332) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-E2F1 (Ser332) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB5097
• Supplier Catalog Number: orb5097
• Alternative Catalog Number: BYT-ORB5097-100,BYT-ORB5097-200,BYT-ORB5097-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human E2F1 around the phosphorylation site of Ser332 IV(p-S)PP
• Conjugation: Unconjugated
• Alternative Names: phospho-E2F1(Ser332), p-E2F1(phospho-Ser332), E2F1(phospho S332), E2F 1, E2F transcription factor 1, E2F-1, E2f1 E2F transcription factor 1, KIAA4009, mKIAA4009, OTTHUMP00000030661, PBR 3, PBR3, PRB binding protein E2F 1, PRB-binding protein E2F-1, RBAP 1, RBAP-1, RBAP1, RBBP 3, RBBP-3, RBBP3, RBP 3, RBP3, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1, E2F1_HUMAN.

Product Description

Phospho-E2F1 (Ser332) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 46 kDa
• UniProt: Q01094
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: E2F1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Application Notes: Modification: Phosphorylated

Images

Immunohistochemical staining of mouse intestine tissue using E2F1 (phospho-Ser332) antibody.

Immunohistochemical staining of human colon cancer tissue using E2F1 (phospho-Ser332) antibody.

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-phospho-E2F1 (Ser332) antibody (orb5097), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-E2F1 (Ser332)) Polyclonal Antibody, Unconjugated (orb5097) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Hela cell lysates, Lane 2: Human A673 cell lysates, Lane 3: Human Molt-4 cell lysates, Lane 4: Human U251cell lysates, Lane 5: Human HepG2 cell lysates, Primary: Anti-phospho-E2F1 (Ser332) (orb5097) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 61 kDa.

Tissue/Cell: human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-E2F1 (Ser332) Polyclonal Antibody, Unconjugated (orb5097) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human skin tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-E2F1 (Ser332) Polyclonal Antibody, Unconjugated (orb5097) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-E2F1 (Ser332) Polyclonal Antibody, Unconjugated (orb5097) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.