Application Notes: This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (alpha chain) and C-terminal fragment (partial beta chain) with calculated MW of 81.7 kDa and 24.3 kDa respectively. The protein migrates as 45-55 kDa (partial beta chain), 100-125 kDa (alpha chain) and 150 kDa (alpha chain & partial beta chain) under reducing (R) condition (SDS-PAGE) due to glycosylation
Immobilized Mouse IGF-I R, His Tag (Cat. No. orb511248) at 5 µg/mL (100 µL/well) can bind Human IGF-I, Fc Tag (Cat. No. orb257564) with a linear range of 7.8-125 ng/mL (QC tested).
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Mouse IGF-I R / CD221 Protein.
SDS-Page analysis of Mouse IGF-I R / CD221 Protein.
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