The Nectin 4 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Nectin 4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Application Dilute:
WB: 1:500-1000
Western blot analysis of Nectin 4 expression in Hela (A), MCF7 (B), MEF (C), PC12 (D) whole cell lysates. (Predicted band size: 55 kD, Observed band size: 52, 40, 66 kD)
Immunohistochemical analysis of Nectin 4 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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