Phospho-ERK5 (Ser731+Thr733) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-ERK5 (Ser731+Thr733) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB5184
• Supplier Catalog Number: orb5184
• Alternative Catalog Number: BYT-ORB5184-50,BYT-ORB5184-100,BYT-ORB5184-200
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ERK5 around the phosphorylation site of Ser731+Thr733 VF(p-S)G(p-T)PK
• Conjugation: Unconjugated
• Alternative Names: ERK5(phospho Ser731+Thr733), Big MAP kinase 1, BMK 1, BMK 1 kinase, BMK-1, BMK1, BMK1 Kinase, EC 2.7.11.24, ERK 4, ERK 5, ERK-5, ERK4, Extracellular Signal Regulated Kinase 5, Extracellular signal-regulated kinase 5, MAP kinase 7, MAPK 7, MAPK7, Mitogen Activated Protein Kinase 7, Mitogen-activated protein kinase 7, MK07_HUMAN, OTTHUMP00000065906, OTTHUMP00000065907, PRKM 7, PRKM7, PROTEIN KINASE, MITOGEN-ACTIVATED, 7.

Product Description

Phospho-ERK5 (Ser731+Thr733) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 90 kDa
• UniProt: Q13164
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: MAPK7

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

Immunohistochemical staining of human myometrium tissue using ERK5 (phospho-Ser731-Thr733) antibody.

Immunohistochemical staining of human kidney tissue using ERK5 (phospho-Ser731-Thr733) antibody.

Paraformaldehyde-fixed, paraffin embedded (Human breast cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-ERK5 (Ser731+Thr733)) Polyclonal Antibody, Unconjugated (orb5184) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-ERK5 (Ser731+Thr733)) Polyclonal Antibody, Unconjugated (orb5184) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-ERK5 (Ser731+Thr733)) Polyclonal Antibody, Unconjugated (orb5184) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Heart tissue lysates, Lane 4: Rat Muscle tissue lysates, Lane 5: Rat Cerebrum tissue lysates, Primary: Anti-phospho-ERK5 (Ser731+Thr733) (orb5184) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90 kDa, Observed band size: 59 kDa.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-ERK5 (Ser731+Thr733) Polyclonal Antibody, FITC conjugated 1:200, used at 1:200 dilution for 40 minutes at 37C.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-ERK5 (Ser731+Thr733) Polyclonal Antibody, Unconjugated (orb5184) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C.