AIF/AIFM1 Mouse Monoclonal Antibody, Clone: [2I5], Unconjugated

Product Overview

• Article Name: AIF/AIFM1 Mouse Monoclonal Antibody, Clone: [2I5], Unconjugated
• Biozol Catalog Number: BYT-ORB547790
• Supplier Catalog Number: orb547790
• Alternative Catalog Number: BYT-ORB547790-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: FC, ICC, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human AIF, identical to the related mouse and rat sequences.
• Conjugation: Unconjugated
• Alternative Names: AIFM1 antibody, AIFM1_HUMAN antibody, Apoptosis inducing factor 1, mitochondrial antibody, Apoptosis inducing factor antibody, Apoptosis inducing factor, mitochondrion associated, 1 antibody, Apoptosis-inducing factor 1 antibody, CMTX4 antibody, COXPD6 antibody, Harlequin antibody, Hq antibody, mAIF antibody, MGC111425 antibody, MGC5706 antibody, mitochondrial antibody, Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody, neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody, PDCD 8 antibody, PDCD8 antibody, Programmed cell death 8 (apoptosis inducing factor) antibody, Programmed cell death 8 antibody, Programmed cell death 8 isoform 1 antibody, Programmed cell death 8 isoform 2 antibody, Programmed cell death 8 isoform 3 antibody, Programmed cell death protein 8 antibody, Programmed cell death protein 8 mitochondrial antibody, Programmed cell death protein 8 mitochondrial precursor antibody, Striatal apoptosis inducing factor antibody

Product Description

Anti-AIF/AIFM1 Antibody (monoclonal, 2I5). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Properties

• Clonality: Monoclonal
• Concentration: 500 µg/ml
• Clone Designation: [2I5]
• Molecular Weight: 70 kDa
• UniProt: O95831
• Buffer: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Form: Lyophilized
• Target: Apoptosis-inducing factor 1, mitochondrial

Application Notes

• Application Dilute: Western blot, 0.1-0.5µg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml Immunofluorescence, 2µg/ml Immunocytochemistry/Immunofluorescence, 5µg/ml Flow Cytometry (Fixed), 1-3µg/1x10 6 cells

Images

Flow Cytometry analysis of Raji cells using anti-AIF antibody. Overlay histogram showing Raji cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AIF Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-mouse IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

IF analysis of AIF using anti-AIF antibody. AIF was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL mouse anti-AIF Antibody overnight at 4C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of AIF using anti-AIF antibody. AIF was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/mL mouse anti-AIF Antibody overnight at 4C. DyLight550 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of AIF using anti-AIF antibody. AIF was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-AIF Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of AIF using anti-AIF antibody. AIF was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-AIF Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of AIF using anti-AIF antibody. AIF was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-AIF Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of AIF using anti-AIF antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates, Lane 2: Raji whole cell lysates, Lane 3: PC-3 whole cell lysates, Lane 4: Hela